New mechanisms of phenytoin in calcium homeostasis: competitive inhibition of CD38 in hippocampal cells.

ABSTRACT:

Purpose

Phenytoin is a major anticonvulsant drug that is effective to improve arrhythmia and neuropathic pain. According to early works, phenytoin affected cell membrane depolarization by sodium channel blocking, guanylyl and adenylyl cyclase suppression that cause to intracellular Na⁺ and Ca²⁺ downregulation. This study was aimed to clarify some ambiguities in pathophysiological action of phenytoin by in vitro and molecular docking analyses.

Methods

In this study intracellular free Ca²⁺ of primary culture of embryonic mouse hippocampus evaluated via Fura 2 as fluorescent probe. The effects of phenytoin on ADP ribosyl cyclase activity was assessed by recently developed fluorometric assay. Molecular docking simulation was also implemented to i

SUBMITTER: Sadeghi L 

PROVIDER: S-EPMC6279657 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature