Differential regulation of CD4⁺ T cell subsets by Silymarin in vitro and in ovalbumin immunized mice.

ABSTRACT: CD4⁺ T cell subsets including regulatory T cells (Tregs), Th1 and Th17 are critical for control and development of inflammation and autoimmunity. We investigated the in vitro and in vivo effects of silymarin, a well-known herbal medicine on differentiation and function of Tregs and Th1 and Th17 responses. For in vitro study, mice splenocytes treated with 20-30 μg/ml silymarin were evaluated for gene expressions of specific transcription factors and cytokines of CD4⁺ T cell subsets using real-time PCR. Induction of Treg cell development in the presence of silymarin was performed on isolated naïve CD4⁺ T cells. Effect of silymarin-induced Tregs on T cell suppression was determined by CFSE labeling method. Results of this part showed that silymarin significant

SUBMITTER: Namdari H 

PROVIDER: S-EPMC6279658 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature