An Organoid-Based Preclinical Model of Human Gastric Cancer.

ABSTRACT:

Background & aims

Our goal was to develop an initial study for the proof of concept whereby gastric cancer organoids are used as an approach to predict the tumor response in individual patients.

Methods

Organoids were derived from resected gastric cancer tumors (huTGOs) or normal stomach tissue collected from sleeve gastrectomies (huFGOs). Organoid cultures were treated with standard-of-care chemotherapeutic drugs corresponding to patient treatment: epirubicin, oxaliplatin, and 5-fluorouracil. Organoid response to chemotherapeutic treatment was correlated with the tumor response in each patient from whom the huTGOs were derived. HuTGOs were orthotopically transplanted into the gastric mucosa of NOD scid gamma mice.

Results

Whereas huFGOs exhibited a half maximal inhib

SUBMITTER: Steele NG 

PROVIDER: S-EPMC6279812 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature