Biochemical Characterization of <i>Aspergillus fumigatus</i> AroH, a Putative Aromatic Amino Acid Aminotransferase.

ABSTRACT: The rise in the frequency of nosocomial infections is becoming a major problem for public health, in particular in immunocompromised patients. Aspergillus fumigatus is an opportunistic fungus normally present in the environment directly responsible for lethal invasive infections. Recent results suggest that the metabolic pathways related to amino acid metabolism can regulate the fungus-host interaction and that an important role is played by enzymes involved in the catabolism of L-tryptophan. In particular, in A. fumigatus L-tryptophan regulates Aro genes. Among them, AroH encodes a putative pyridoxal 5'-phosphate-dependent aminotransferase. Here we analyzed the biochemical features of recombinant purified AroH by spectroscopic and kinetic analyses corroborated by i

SUBMITTER: Dindo M 

PROVIDER: S-EPMC6279937 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature