The Effect of Smoking Behavior on Alveolar Bone Marrow Mesenchymal Stem Cells of Clinical Implant Patient.

ABSTRACT:

Objective

The hazardous effects of smoking on the alveolar bone healing after implant surgery and nicotine on the biofunction of human alveolar bone marrow mesenchymal stem cells (hABMMSCs) were reported. There was little direct evidence regarding the specific detrimental effects of the smoking on hABMMSCs. The aim of this study was to test the influence of smoking behavior on hABMMSCs and the osseointegration situation after implant surgery.

Methods

hABMMSCs from 6 dental implant patients randomly (3 smokers and 3 nonsmokers) were compared. The cell viability, colony forming unit, and cell cycle were performed to assay proliferation capacity. The Oil Red O staining, Alizarin Red staining, alkaline phosphatase staining and activity, adipogenic and osteogenic gene expressions

SUBMITTER: Zhao X 

PROVIDER: S-EPMC6280244 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature