Microsatellite instability test using peptide nucleic acid probe-mediated melting point analysis: a comparison study.

ABSTRACT:

Background

Analysis of high microsatellite instability (MSI-H) phenotype in colorectal carcinoma (CRC) is important for evaluating prognosis and choosing a proper adjuvant therapy. Although the conventional MSI analysis methods such as polymerase chain reaction (PCR) fragment analysis and immunohistochemistry (IHC) show high specificity and sensitivity, there are substantial barriers to their use.

Methods

In this study, we analyzed the MSI detection performance of three molecular tests and IHC. For the molecular tests, we included a recently developed peptide nucleic acid probe (PNA)-mediated real-time PCR-based method using five quasi-monomorphic mononucleotide repeat markers (PNA method) and two conventional PCR fragment analysis methods using NCI markers (NCI method) or f

SUBMITTER: Jang M 

PROVIDER: S-EPMC6280403 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature