A novel approach for rapid high-throughput selection of recombinant functional rat monoclonal antibodies.

ABSTRACT:

Background

Most monoclonal antibodies against mouse antigens have been derived from rat spleen-mouse myeloma fusions, which are valuable tools for purposes ranging from general laboratory reagents to therapeutic drugs, and yet selecting and expressing them remains a time-consuming and inefficient process. Here, we report a novel approach for the rapid high-throughput selection and expression of recombinant functional rat monoclonal antibodies with different isotypes.

Results

We have developed a robust system for generating rat monoclonal antibodies through several processes involving simultaneously immunizing rats with three different antigens expressing in a mixed cell pools, preparing hybridoma cell pools, in vitro screening and subsequent cloning of the rearranged light a

SUBMITTER: Chen Q 

PROVIDER: S-EPMC6280491 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature