Application of the CRISPR/Cas9 system for modification of flower color in Torenia fournieri.

ABSTRACT:

Background

CRISPR/Cas9 technology is one of the most powerful and useful tools for genome editing in various living organisms. In higher plants, the system has been widely exploited not only for basic research, such as gene functional analysis, but also for applied research such as crop breeding. Although the CRISPR/Cas9 system has been used to induce mutations in genes involved in various plant developmental processes, few studies have been performed to modify the color of ornamental flowers. We therefore attempted to use this system to modify flower color in the model plant torenia (Torenia fournieri L.).

Results

We attempted to induce mutations in the torenia flavanone 3-hydroxylase (F3H) gene, which encodes a key enzyme involved in flavonoid biosynthesis. Application of

SUBMITTER: Nishihara M 

PROVIDER: S-EPMC6280492 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature