CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells.

ABSTRACT:

Background

There is an urgent need to identify new molecular targets for treatment of osteosarcoma. Circular RNAs are a class of endogenous RNAs that are extensively found in mammalian cells and exert critical functions in the regulation of gene expression, but in osteosarcoma the underlying molecular mechanism of circular RNAs remain poorly understood. Here we assessed the tumorigenesis properties of a circular RNA, circFAT1 in osteosarcoma.

Methods

The effects of circFAT1/miR-375/YAP1 was evaluated on human osteosarcoma cells growth, apoptosis, migration, invasion and tumorigenesis. Signaling pathways were analyzed by western blotting, qRT-PCR, fluorescence in situ hybridization, chromogenic in situ hybridization,RNA Binding Protein Immunoprecipitation and immunofluorescen

SUBMITTER: Liu G 

PROVIDER: S-EPMC6280518 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature