Construction and characterization of a synthetic Baculovirus-inducible 39K promoter.

ABSTRACT:

Background

Silkworm genetic engineering is widely used in gene function, silk engineering and disease-resistant engineering in most of Asia. Some of the earliest promoter elements are used to control the development of silkworm transgenic expression and gene therapy. However, the low expression and specificity of natural promoters limit the applications of genetic engineering. To construct a highly efficient synthetic inducible promoter in the Bombyx mori (Lepidoptera), we analyzed the regulatory elements and functional regions of the B. mori nucleopolyhedrovirus 39 K promoter.

Results

Truncated mutation analysis of the 39 K promoter showed that the transcriptional regulatory region spanning positions - 573 to - 274 and + 1 to + 62 are essential for virus-induc

SUBMITTER: Dong ZQ 

PROVIDER: S-EPMC6280533 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature