Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila.

ABSTRACT:

Background

The regulatory mechanisms of hematopoiesis and cellular immunity show a high degree of similarity between insects and mammals, and Drosophila has become a good model for investigating cellular immune responses. Jumeau (Jumu) is a member of the winged-helix/forkhead (FKH) transcription factor family and is required for Drosophila development. Adult jumu mutant flies show defective hemocyte phagocytosis and a weaker defense capability against pathogen infection. Here, we further investigated the role of jumu in the regulation of larval hemocyte development and phagocytosis.

Methods

In vivo phagocytosis assays, immunohistochemistry, Real-time quantitative PCR and immunoblotting were performed to investigate the effect of Jumu on hemocyte phagocytosis. 5-Bromo-2-deoxy

SUBMITTER: Hao Y 

PROVIDER: S-EPMC6280549 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature