Long-read sequencing uncovers a complex transcriptome topology in varicella zoster virus.

ABSTRACT:

Background

Varicella zoster virus (VZV) is a human pathogenic alphaherpesvirus harboring a relatively large DNA molecule. The VZV transcriptome has already been analyzed by microarray and short-read sequencing analyses. However, both approaches have substantial limitations when used for structural characterization of transcript isoforms, even if supplemented with primer extension or other techniques. Among others, they are inefficient in distinguishing between embedded RNA molecules, transcript isoforms, including splice and length variants, as well as between alternative polycistronic transcripts. It has been demonstrated in several studies that long-read sequencing is able to circumvent these problems.

Results

In this work, we report the analysis of the VZV lytic transcrip

SUBMITTER: Prazsak I 

PROVIDER: S-EPMC6280550 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature