Diurnal variation of steroid hormones and their reference intervals using mass spectrometric analysis.

ABSTRACT:

Objective

Accurate measurement of steroid hormones remains challenging. Mass spectrometry affords a reliable means for quantitating steroid profiles accurately. Our objective was to establish and define (1) the extent of diurnal fluctuations in steroid concentrations that potentially necessitate strict adherence to time of sample acquisition and (2) time-dependent steroid reference intervals.

Design

Nine steroid markers were examined in couplets in males and females.

Methods

Using isotope dilution high-performance liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis, we developed a multi-steroid profile requiring only a minimal volume of serum (0.1 mL). Couplet (AM and PM) measurements of steroid hormones for 120 healthy females (F) and 62 healthy males

SUBMITTER: Parikh TP 

PROVIDER: S-EPMC6280590 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature