NanoPARE: parallel analysis of RNA 5' ends from low-input RNA.

ABSTRACT: Diverse RNA 5' ends are generated through both transcriptional and post-transcriptional processes. These important modes of gene regulation often vary across cell types and can contribute to the diversification of transcriptomes and thus cellular differentiation. Therefore, the identification of primary and processed 5' ends of RNAs is important for their functional characterization. Methods have been developed to profile either RNA 5' ends from primary transcripts or the products of RNA degradation genome-wide. However, these approaches either require high amounts of starting RNA or are performed in the absence of paired gene-body mRNA-seq data. This limits current efforts in RNA 5' end annotation to whole tissues and can prevent accurate RNA 5' end classification due to biases in the dat

SUBMITTER: Schon MA 

PROVIDER: S-EPMC6280765 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature