Rapid and highly-specific generation of targeted DNA sequencing libraries enabled by linking capture probes with universal primers.

ABSTRACT: Targeted Next Generation Sequencing (NGS) is being adopted increasingly broadly in many research, commercial and clinical settings. Currently used target capture methods, however, typically require complex and lengthy (sometimes multi-day) workflows that complicates their use in certain applications. In addition, small panels for high sequencing depth applications such as liquid biopsy typically have low on-target rates, resulting in unnecessarily high sequencing cost. We have developed a novel targeted sequencing library preparation method, named Linked Target Capture (LTC), which replaces typical multi-day target capture workflows with a single-day, combined 'target-capture-PCR' workflow. This approach uses physically linked capture probes and PCR primers and is expected to work with pan

SUBMITTER: Pel J 

PROVIDER: S-EPMC6281261 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature