Plasmonic ELISA for Sensitive Detection of Disease Biomarkers with a Smart Phone-Based Reader.

ABSTRACT: Serum myoglobin is one of the earliest markers for the diagnosis of acute myocardial infarction. It is, therefore, critical to develop a point-of-care testing technology for myoglobin detection. In this work, we reported a sensitive plasmonic immunoassay-based on enzyme-mediated localized surface plasmon resonance change of gold nanorods for the point-of-care testing detection of myoglobin. In addition, we developed a novel plasmonic immunoassay reader using the ambient light sensor of smart phone to increase the accessibility and utility of the plasmonic immunoassay. The linear detection range of gold nanorods-based plasmonic immunoassay for myoglobin detection was 0.1-1000 ng mL^-1 and the limit of detection was 0.057 ng mL^-1. Myoglobin in serum samples was also anal

SUBMITTER: Yang Q 

PROVIDER: S-EPMC6281541 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature