Temporal control of gene expression by the pioneer factor Zelda through transient interactions in hubs.

ABSTRACT: Pioneer transcription factors can engage nucleosomal DNA, which leads to local chromatin remodeling and to the establishment of transcriptional competence. However, the impact of enhancer priming by pioneer factors on the temporal control of gene expression and on mitotic memory remains unclear. Here we employ quantitative live imaging methods and mathematical modeling to test the effect of the pioneer factor Zelda on transcriptional dynamics and memory in Drosophila embryos. We demonstrate that increasing the number of Zelda binding sites accelerates the kinetics of nuclei transcriptional activation regardless of their transcriptional past. Despite its known pioneering activities, we show that Zelda does not remain detectably associated with mitotic chromosomes and is neither necessary no

SUBMITTER: Dufourt J 

PROVIDER: S-EPMC6281682 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature