Persistent elevations in circulating INS DNA among subjects with longstanding type 1 diabetes.

ABSTRACT:

Aim

To evaluate whether β cells continue to undergo death in the later stages of type 1 diabetes (T1D).

Materials and methods

Fasting banked sera from a cross-section of 90 participants in the T1D Exchange Registry with longstanding T1D (median duration of 9 years) were analysed. Subjects were determined to be C-peptide (-) or (+) based on mixed-meal tolerance testing. Results were compared with 54 adult non-diabetic controls. Stimulated samples were assayed in a subset of subjects. Levels of unmethylated and methylated preproinsulin (INS) DNA were analysed using digital droplet PCR.

Results

Fasting and stimulated circulating unmethylated INS DNA levels were increased among both C-peptide (-) and C-peptide (+) subjects with longstanding T1D compared with non-diabetic

SUBMITTER: Neyman A 

PROVIDER: S-EPMC6281817 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature