Data on retinoic acid and reduced serum concentration induced differentiation of Neuro-2a neuroblastoma cells.

ABSTRACT: The present data describe the relative neuro-2a cellular differentiation induced by reducing serum concentration (0.1% FBS) in DMEM in the presence/absence of 20 μM retinoic acid (RA). Neurite outgrowth was observed within 24 h in DMEM supplemented with reduced serum and retinoic acid (GpIV). The CFSE based proliferation assay data signified cessation of neuro-2a cellular proliferation in GpIV. An increase in the number of cells arrested at G₀/G₁ phase was also evident in GpIV and DMEM supplemented with 0.1% FBS (GpIII). Moreover, GpIV cells had improved mRNA and protein expression of Rbfox3/NeuN and choline acetyltransferase (ChAT).

SUBMITTER: Kumar M 

PROVIDER: S-EPMC6282191 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature