Molecular characterization of a 31 kDa protein from Trichinella spiralis and its induced immune protection in BALB/c mice.

ABSTRACT:

Background

Trichinella spiralis is an important foodborne zoonotic parasite and it is necessary to develop a vaccine in order to interrupt transmission from animals to humans. A 31 kDa protein from T. spiralis (Ts31) is an antigen targeted by protective antibodies, and Ts31 contains a domain of trypsin-like serine protease that might have the function of serine protease. The purpose of this study was to investigate the molecular characteristics of Ts31 and its induced immune protection.

Methods

Expression and localization of Ts31 in various T. spiralis phases were investigated using qPCR and immunofluorescent test (IFT). The specific binding between Ts31 and intestinal epithelium cells (IECs) was analyzed by Far-Western blotting, ELISA and IFT, and the cellular localization

SUBMITTER: Ren HN 

PROVIDER: S-EPMC6282284 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature