EZH2 variants differentially regulate polycomb repressive complex 2 in histone methylation and cell differentiation.

ABSTRACT:

Background

Polycomb repressive complex 2 (PRC2) is responsible for establishing and maintaining histone H3K27 methylation during cell differentiation and proliferation. H3K27 can be mono-, di-, or trimethylated, resulting in differential gene regulation. However, it remains unknown how PRC2 specifies the degree and biological effects of H3K27 methylation within a given cellular context. One way to determine PRC2 specificity may be through alternative splicing of Ezh2, PRC2's catalytic subunit, during cell differentiation and tissue maturation.

Results

We fully characterized the alternative splicing of Ezh2 in somatic cells and male germ cells and found that Ezh's exon 14 was differentially regulated during mitosis and meiosis. The Ezh2 isoform containing exon 14 (ex14-Ezh2)

SUBMITTER: Mu W 

PROVIDER: S-EPMC6282306 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature