Isolation and characterization of camelid single-domain antibodies against HER2.

ABSTRACT:

Objective

To isolate and characterize novel high-affinity llama single-domain antibodies against human HER2.

Results

We immunized a llama with human HER2, constructed a phage-displayed V_HH library from the lymphocytes of the animal, and isolated six unique HER2-specific V_HHs by panning. All six V_HHs were unique at the amino acid level and were clonally unrelated, as reflected by their distinct CDR3 lengths. All six V_HHs recognized recombinant human HER2 ectodomain with monovalent affinities ranging from 1 to 51 nM, had comparable affinities for cynomolgus monkey HER2, and bound HER2⁺ SKOV3 cells by flow cytometry. Three of the V_HHs recognized recombinant murine HER2 with no loss of affinity compared with human

SUBMITTER: Hussack G 

PROVIDER: S-EPMC6282393 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature