Large-scale, cross-flow based isolation of highly pure and endocytosis-competent extracellular vesicles.

ABSTRACT: Isolation of extracellular vesicles (EVs) from cell culture supernatant or plasma can be accomplished in a variety of ways. Common measures to quantify relative success are: concentration of the EVs, purity from non-EVs associated protein, size homogeneity and functionality of the final product. Here, we present an industrial-scale workflow for isolating highly pure and functional EVs using cross-flow based filtration coupled with high-molecular weight Capto Core size exclusion. Through this combination, EVs loss is kept to a minimum. It outperforms other isolation procedures based on a number of biochemical and biophysical assays. Moreover, EVs isolated through this method can be further concentrated down or directly immunopurified to obtain discreet populations of EVs. From our results,

SUBMITTER: McNamara RP 

PROVIDER: S-EPMC6282418 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature