Selection of fast and slow growing bacteria from fecal microbiota using continuous culture with changing dilution rate.

ABSTRACT: Background: Nutrient and energy metabolism in human colon depends on bacterial growth rate that is determined by the colonic transit rate. Objective: A novel approach, De-stat culture was used to distinguish the fast and slow growing sub-populations from fecal microbiota. Design: The enrichment and metabolism of bacteria from pooled fecal cultures of children was studied at dilution rates D = 0.2-0.0 1/h in mucin-supplemented media containing either arabinogalactan or apple pectin. Results: The study revealed clear differentiation of the fecal microbiota at higher (above 0.1 1/h) and lower (below 0.1 1/h) dilution rates, along with metabolic changes. Similarity of the fast and slow growing bacteria was observed in two different fecal pools and on both sub

SUBMITTER: Adamberg K 

PROVIDER: S-EPMC6282430 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature