Eccrine Sweat as a Biofluid for Profiling Immune Biomarkers.

ABSTRACT:

Purpose

Sweat is a relatively unexplored biofluid for diagnosis and monitoring of disease states. In this study, the proteomic profiling of immune-related biomarkers from healthy individuals are presented.

Experimental design

Eccrine sweat samples are collected from 50 healthy individuals. LC-MS/MS is performed on two pools of sweat samples from five male and female participants. Individual sweat samples are analyzed by antibody isotyping microarrays (n = 49), human cytokine arrays (n = 30), and quantitative ELISAs for interleukin-1α (n = 16), epidermal growth factor (n = 6), and angiogenin (n = 7).

Results

In sweat, 220 unique proteins are identified by shotgun analysis. Detectable antibody isotypes include IgA (100% positive; median 1230 ± 28 700 pg mL^-1

SUBMITTER: Katchman BA 

PROVIDER: S-EPMC6282813 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature