W-Type ions formed by electron transfer dissociation of Cys-containing peptides investigated by infrared ion spectroscopy.

ABSTRACT: In mass spectrometry-based peptide sequencing, electron transfer dissociation (ETD) and electron capture dissociation (ECD) have become well-established fragmentation methods complementary to collision-induced dissociation. The dominant fragmentation pathways during ETD and ECD primarily involve the formation of c- and z^• -type ions by cleavage of the peptide backbone at the N─C_α bond, although neutral losses from amino acid side chains have also been observed. Residue-specific neutral side chain losses provide useful information when conducting database searching and de novo sequencing. Here, we use a combination of infrared ion spectroscopy and quantum-chemical calculations to assign the structures of two ETD-generated w-type fragment ions. These ions are spontaneou

SUBMITTER: Kempkes LJM 

PROVIDER: S-EPMC6283004 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature