Diurnal Variation in PDK4 Expression Is Associated With Plasma Free Fatty Acid Availability in People.

ABSTRACT:

Context

Many biological pathways involved in regulating substrate metabolism display rhythmic oscillation patterns. In rodents, clock genes regulate circadian rhythms of metabolic genes and substrate metabolism. However, the interrelationships among substrate metabolism, metabolic genes, and clock genes have not been fully explored in people.

Objective

We tested the hypothesis that the diurnal expression pattern of pyruvate dehydrogenase kinase 4 (PDK4), a key metabolic enzyme involved in fuel switching between glucose and free fatty acids (FFAs), is associated with plasma FFA concentration and clock genes.

Design and methods

We analyzed peripheral blood mononuclear cells (PBMCs), subcutaneous adipose tissue, and plasma samples obtained serially during 24 hours from m

SUBMITTER: Yamaguchi S 

PROVIDER: S-EPMC6283414 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature