An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.

ABSTRACT: Metabolite-protein interactions define the output of metabolic pathways and regulate many cellular processes. Although diseases are often characterized by distortions in metabolic processes, efficient means to discover and study such interactions directly in cells have been lacking. A stringent implementation of proteome-wide Cellular Thermal Shift Assay (CETSA) was developed and applied to key cellular nucleotides, where previously experimentally confirmed protein-nucleotide interactions were well recaptured. Many predicted, but never experimentally confirmed, as well as novel protein-nucleotide interactions were discovered. Interactions included a range of different protein families where nucleotides serve as substrates, products, co-factors or regulators. In cells exposed to thymidine,

SUBMITTER: Lim YT 

PROVIDER: S-EPMC6283526 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature