Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition.

ABSTRACT: Maternal-to-zygotic transition (MZT) is essential for the formation of a new individual, but is still poorly understood despite recent progress in analysis of gene expression and DNA methylation in early embryogenesis. Dynamic histone modifications may have important roles in MZT, but direct measurements of chromatin states have been hindered by technical difficulties in profiling histone modifications from small quantities of cells. Recent improvements allow for 500 cell-equivalents of chromatin per reaction, but require 10,000 cells for initial steps or require a highly specialized microfluidics device that is not readily available. We developed a micro-scale chromatin immunoprecipitation and sequencing (μChIP-seq) method, which we used to profile genome-wide histone H3 lysine methylatio

SUBMITTER: Dahl JA 

PROVIDER: S-EPMC6283663 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature