Single-molecule imaging correlates decreasing nuclear volume with increasing TF-chromatin associations during zebrafish development.

ABSTRACT: Zygotic genome activation (ZGA), the onset of transcription after initial quiescence, is a major developmental step in many species, which occurs after ten cell divisions in zebrafish embryos. How transcription factor (TF)-chromatin interactions evolve during early development to support ZGA is largely unknown. We establish single molecule tracking in live developing zebrafish embryos using reflected light-sheet microscopy to visualize two fluorescently labeled TF species, mEos2-TBP and mEos2-Sox19b. We further develop a data acquisition and analysis scheme to extract quantitative information on binding kinetics and bound fractions during fast cell cycles. The chromatin-bound fraction of both TFs increases during early development, as expected from a physical model of TF-chromatin interact

SUBMITTER: Reisser M 

PROVIDER: S-EPMC6283880 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature