Assessment of in vitro particle dosimetry models at the single cell and particle level by scanning electron microscopy.

ABSTRACT:

Background

Particokinetic models are important to predict the effective cellular dose, which is key to understanding the interactions of particles with biological systems. For the reliable establishment of dose-response curves in, e.g., the field of pharmacology and toxicology, mostly the In vitro Sedimentation, Diffusion and Dosimetry (ISDD) and Distorted Grid (DG) models have been employed. Here, we used high resolution scanning electron microscopy to quantify deposited numbers of particles on cellular and intercellular surfaces and compare experimental findings with results predicted by the ISDD and DG models.

Results

Exposure of human lung epithelial A549 cells to various concentrations of differently sized silica particles (100, 200 and 500 nm) revealed a remarkably hig

SUBMITTER: Kowoll T 

PROVIDER: S-EPMC6284276 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature