In situ administration of cytokine combinations induces tumor regression in mice.

ABSTRACT:

Background

Recent advances in cancer immunotherapy suggest a possibility of harnessing the immune system to defeat malignant tumors, but the complex immunosuppressive microenvironment confines the therapeutic benefits to a minority of patients with solid tumors.

Methods

A lentivector-based inducible system was established to evaluate the therapeutic effect of cytokines in established tumors. Intratumoral injection of certain cytokine combination in syngeneic tumor models was conducted to assess the therapeutic potentials.

Findings

Doxycycline (Dox)-induced local expression of cytokine combinations exhibites a strong synergistic effect, leading to complete regression of tumors. Notably, IL12 + GMCSF+IL2 expression induces eradication of tumors in all mice tolerated wit

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6284351 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature