Targeting hepatic miR-221/222 for therapeutic intervention of nonalcoholic steatohepatitis in mice.

ABSTRACT:

Background

Effective targeting therapies for common chronic liver disease nonalcoholic steatohepatitis (NASH) are in urgent need. MicroRNA-targeted therapeutics would be potentially an effective treatment strategy of hepatic diseases. Here we investigated the functional role of miR-221/222 and the therapeutic effects of antimiRs-221/222 in NASH mouse models.

Methods

We generated the miR-221/222^flox/flox mice on a C57BL/6 J background and the hepatic miR-221/222 knockout (miR-221/222-LKO) mice. The mice were challenged with the methionine and choline deficient diet (MCDD) or chronic carbon tetrachloride (CCl₄) treatment to generate experimental steatohepatitis models. Adenovirus-mediated re-expression of miR-221/222 was performed on the MCDD-fed miR-221/

SUBMITTER: Jiang X 

PROVIDER: S-EPMC6284352 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature