Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei.

ABSTRACT:

Background

Enterocytozoon hepatopenaei (EHP) is a newly emerged microsporidian parasite that causes retarded shrimp growth in many countries. But there are no effective approaches to control this disease to date. The EHP could be an immune risk factor for increased dissemination of other diseases. Further, EHP infection involves the absence of obvious clinical signs and it is difficult to identify the pathogen through visual examination, increasing the risk of disease dissemination. It is urgent and necessary to develop a specific, rapid and sensitive EHP-infected shrimp diagnostic method to detect this parasite. In the present study, we developed and evaluated a rapid real-time loop-mediated isothermal amplification (real-time LAMP) for detection of EHP.

Methods

A ra

SUBMITTER: Cai SX 

PROVIDER: S-EPMC6284447 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature