Simultaneous dual-channel imaging to quantify interdependent protein recruitment to laser-induced DNA damage sites.

ABSTRACT: Fluorescence microscopy in combination with the induction of localized DNA damage using focused light beams has played a major role in the study of protein recruitment kinetics to DNA damage sites in recent years. Currently published methods are dedicated to the study of single fluorophore/single protein kinetics. However, these methods may be limited when studying the relative recruitment dynamics between two or more proteins due to cell-to-cell variability in gene expression and recruitment kinetics, and are not suitable for comparative analysis of fast-recruiting proteins. To tackle these limitations, we have established a time-lapse fluorescence microscopy method based on simultaneous dual-channel acquisition following UV-A-induced local DNA damage coupled with a standardized image and

SUBMITTER: Garbrecht J 

PROVIDER: S-EPMC6284507 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature