Organotypic microfluidic breast cancer model reveals starvation-induced spatial-temporal metabolic adaptations.

ABSTRACT:

Background

Ductal carcinoma in situ (DCIS) is the earliest stage of breast cancer. During DCIS, tumor cells remain inside the mammary duct, growing under a microenvironment characterized by hypoxia, nutrient starvation, and waste product accumulation; this harsh microenvironment promotes genomic instability and eventually cell invasion. However, there is a lack of biomarkers to predict what patients will transition to a more invasive tumor or how DCIS cells manage to survive in this harsh microenvironment.

Methods

In this work, we have developed a microfluidic model that recapitulates the DCIS microenvironment. In the microdevice, a DCIS model cell line was grown inside a luminal mammary duct model, embedded in a 3D hydrogel with mammary fibroblasts. Cell behavior was monito

SUBMITTER: Ayuso JM 

PROVIDER: S-EPMC6284542 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature