A systematic approach for analysis and characterization of mispairing in bispecific antibodies with asymmetric architecture.

ABSTRACT: Immunoglobulin G-like bispecific antibodies with asymmetric architecture are among the most widely used bispecific antibody formats for diagnostic and therapeutic applications. The primary technical challenge for this format is how to achieve correctly paired assembly of four unique polypeptide chains. Advances in protein engineering and process development are being used to overcome these challenges and are driving a corresponding demand for sensitive analytical tools to monitor and control mispaired species. Here, we report a systematic approach for analysis and characterization of mispairing in asymmetric bispecific antibodies. This approach consists of three orthogonal components, the first of which is a liquid chromatography (LC)-mass spectrometry (MS)-based method to measure the mass

SUBMITTER: Wang C 

PROVIDER: S-EPMC6284573 | biostudies-literature | 2018 Nov-Dec

REPOSITORIES: biostudies-literature