Prevalence of the integration status for human papillomavirus 16 in esophageal carcinoma samples.

ABSTRACT:

Background/aims

To investigate the etiology of esophageal cancer (EC) related with human papillomavirus (HPV) infection.

Materials and methods

Fresh surgically resected tissue samples and clinical information were obtained from 189 patients. Genomic DNA was extracted, and HPV was detected using polymerase chain reaction (PCR) with HPV L1 gene primers of MY09/11; HPV16 was detected using HPV16 E6 type-specific primer sets. Copies of HPV16 E2, E6, and the human housekeeping gene β-actin were tested using quantitative PCR to analyze the relationship between HPV16 integration and esophageal squamous cell carcinoma and the relationship between the HPV16 integration status and clinical information of patients.

Results

Of the 189 samples, 168 HPV-positive samples were detect

SUBMITTER: Li S 

PROVIDER: S-EPMC6284706 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature