Absence of genetic selection in a pathogenic Escherichia coli strain exposed to the manure-amended soil environment.

ABSTRACT: Escherichia coli that express curli are more common in subsurface soil drainage when manure is surface applied. However, it is unknown whether this arises from mutations in individual strains leading to curli expression or by selection for individuals already expressing higher levels of curli. To test this, we examined curli production in pathogenic E. coli O157:H7 EDL933 as a function of manure management. Five treatments were investigated: (1) soil only, (2) soil with surface-applied E. coli O157:H7 EDL933 Δstx1-2 (EcO157), (3) soil with incorporated EcO157, (4) soil with surface-applied EcO157-inoculated manure, and (5) soil with incorporated EcO157-inoculated manure. EcO157 was reisolated from soils immediately after application and weekly thereafter for 8 weeks. EcO157 in the surface-

SUBMITTER: Truhlar AM 

PROVIDER: S-EPMC6286177 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature