Noninvasive and quantitative measurement of C4d deposition for the diagnosis of antibody-mediated cardiac allograft rejection.

ABSTRACT:

Background

C4d is a specific biomarker for the diagnosis of antibody-mediated rejection (AMR) after cardiac transplantation. Although strongly recommended, routine C4d surveillance is hindered by the invasive nature of endomyocardial biopsy. Targeted ultrasound (US) has high sensitivity, and C4d is abundantly expressed within the graft of patients experiencing AMR, which makes it possible to visualize C4d deposition in vivo using targeted US.

Methods

We designed a serial dilution of C4d-targeted microbubbles (MB_C4d) using a streptavidin-biotin conjugation system. A rat model of AMR with C4d deposition was established by pre-sensitization with skin transplantation before cardiac transplantation. MB_C4d were injected into recipients and then qualitatively

SUBMITTER: Liao T 

PROVIDER: S-EPMC6286270 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature