A high-fat diet alters genome-wide DNA methylation and gene expression in SM/J mice.

ABSTRACT:

Background

While the genetics of obesity has been well defined, the epigenetics of obesity is poorly understood. Here, we used a genome-wide approach to identify genes with differences in both DNA methylation and expression associated with a high-fat diet in mice.

Results

We weaned genetically identical Small (SM/J) mice onto a high-fat or low-fat diet and measured their weights weekly, tested their glucose and insulin tolerance, assessed serum biomarkers, and weighed their organs at necropsy. We measured liver gene expression with RNA-seq (using 21 total libraries, each pooled with 2 mice of the same sex and diet) and DNA methylation with MRE-seq and MeDIP-seq (using 8 total libraries, each pooled with 4 mice of the same sex and diet). There were 4356 genes with expression

SUBMITTER: Keleher MR 

PROVIDER: S-EPMC6286549 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature