Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment.

ABSTRACT:

Background

Approximately 500 Tg of isoprene are emitted to the atmosphere annually, an amount similar to that of methane, and despite its significant effects on the climate, very little is known about the biological degradation of isoprene in the environment. Isolation and characterisation of isoprene degraders at the molecular level has allowed the development of probes targeting isoA encoding the α-subunit of the isoprene monooxygenase. This enzyme belongs to the soluble diiron centre monooxygenase family and catalyses the first step in the isoprene degradation pathway. The use of probes targeting key metabolic genes is a successful approach in molecular ecology to study specific groups of bacteria in complex environments. Here, we developed and tested a novel isoA PCR primer set

SUBMITTER: Carrion O 

PROVIDER: S-EPMC6286570 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature