Noncanonical farnesoid X receptor signaling inhibits apoptosis and impedes liver fibrosis.

ABSTRACT:

Background

Hepatocyte is particularly vulnerable to apoptosis, a hallmark of many liver diseases. Although pro-apoptotic mechanisms have been extensively explored, less is known about the hepatocyte-specific anti-apoptotic molecular events and it lacks effective approach to combat hepatocyte apoptosis. We investigated the anti-apoptotic effect and mechanism of farnesoid X receptor (FXR), and strategies of how to target FXR for inhibiting apoptosis implicated in liver fibrosis.

Methods

Sensitivity to apoptosis was compared between wild type and Fxr^-/- mice and in cultured cells. Cell-based and cell-free assays were employed to identify the binding protein of FXR and to uncover the mechanism of its anti-apoptotic effect. Overexpression of FXR by adenovirus-FXR was e

SUBMITTER: Wang H 

PROVIDER: S-EPMC6286639 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature