Salvianolic acid A targeting the transgelin-actin complex to enhance vasoconstriction.

ABSTRACT:

Background

Salvia miltiorrhiza is used extensively to treat cardiovascular diseases. SAA is a major bioactive component in Salvia miltiorrhiza and mediates myocardial ischemia (MI). However, the industrial production of SAA is limited due to low yields. In addition, the direct targets of SAA are unknown. Here we explore cardioprotective mechanisms and targets of SAA in the cardiovascular system.

Methods

Transgelin and actin were identified as targets of SAA using a chemical biology method and were validated by Biacore analysis, microscale thermophoresis and single-molecule imaging. Studies of transgelin (-/-) knockout mice further verify the target. Cardioprotective mechanisms and targets of SAA were studied in cultured vascular smooth muscle cells and transgenic mice.

Fi

SUBMITTER: Zhong W 

PROVIDER: S-EPMC6286650 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature