Transcriptional Landscape of a bla _KPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10.

ABSTRACT: The diffusion of KPC-2 carbapenemase is closely related to the spread of Klebsiella pneumoniae of the clonal-group 258 and linked to IncFII_K plasmids. Little is known about the biology of multi-drug resistant plasmids and the reasons of their successful dissemination. Using E. coli TOP10 strain harboring a multi-replicon IncFII_K-IncFIB bla _KPC-2-gene carrying plasmid pBIC1a from K. pneumoniae ST-258 clinical isolate BIC-1, we aimed to identify basal gene expression and the effects of imipenem exposure using whole transcriptome approach by RNA sequencing (RNA-Seq). Independently of the antibiotic pressure, most of the plasmid-backbone genes were expressed at low levels. The most expressed pBIC1a genes were involved in antibiotic r

SUBMITTER: Jousset AB 

PROVIDER: S-EPMC6286996 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature