Characterisation of plasmid-mediated rmtB-1 in Enterobacteriaceae clinical isolates from Sao Paulo, Brazil.

ABSTRACT: OBJECTIVES The emergence of 16S rRNA methyltranferases (16 RMTAses) has jeopardised the clinical use of aminoglycosides. RmtB is one of the most frequently reported in Gram-negatives worldwide. In this study, we aimed to estimate the frequency of 16S RMTAses encoding genes in Enterobacteriaceae isolated in a three-month period from a tertiary Brazilian hospital. METHODS All Gram-negatives classified as resistant to amikacin, gentamicin, and tobramycin by agar screening were selected for analysis. The presence of 16SRMTases encoding genes was verified by polymerase chain reaction (PCR). Antimicrobial susceptible profile was determined by broth microdilution. The genetic relationship among these isolates was accessed by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (

SUBMITTER: Cassu-Corsi D 

PROVIDER: S-EPMC6287189 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature