Transcriptional and epigenetic profiling of nutrient-deprived cells to identify novel regulators of autophagy.

ABSTRACT: Macroautophagy (hereafter autophagy) is a lysosomal degradation pathway critical for maintaining cellular homeostasis and viability, and is predominantly regarded as a rapid and dynamic cytoplasmic process. To increase our understanding of the transcriptional and epigenetic events associated with autophagy, we performed extensive genome-wide transcriptomic and epigenomic profiling after nutrient deprivation in human autophagy-proficient and autophagy-deficient cells. We observed that nutrient deprivation leads to the transcriptional induction of numerous autophagy-associated genes. These transcriptional changes are reflected at the epigenetic level (H3K4me3, H3K27ac, and H3K56ac) and are independent of autophagic flux. As a proof of principle that this resource can be used to identify nove

SUBMITTER: Peeters JGC 

PROVIDER: S-EPMC6287694 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature