Automated phosphopeptide enrichment from minute quantities of frozen malignant melanoma tissue.

ABSTRACT: To acquire a deeper understanding of malignant melanoma (MM), it is essential to study the proteome of patient tissues. In particular, phosphoproteomics of MM has become of significant importance because of the central role that phosphorylation plays in the development of MM. Investigating clinical samples, however, is an extremely challenging task as there is usually only very limited quantities of material available to perform targeted enrichment approaches. Here, an automated phosphopeptide enrichment protocol using the AssayMap Bravo platform was applied to MM tissues and assessed for performance. The strategy proved to be highly-sensitive, less prone to variability, less laborious than existing techniques and adequate for starting quantities at the microgram level. An Fe(III)-NTA-IMAC

SUBMITTER: Murillo JR 

PROVIDER: S-EPMC6287822 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature