A High-Throughput Screen Identifies DYRK1A Inhibitor ID-8 that Stimulates Human Kidney Tubular Epithelial Cell Proliferation.

ABSTRACT:

Background

The death of epithelial cells in the proximal tubules is thought to be the primary cause of AKI, but epithelial cells that survive kidney injury have a remarkable ability to proliferate. Because proximal tubular epithelial cells play a predominant role in kidney regeneration after damage, a potential approach to treat AKI is to discover regenerative therapeutics capable of stimulating proliferation of these cells.

Methods

We conducted a high-throughput phenotypic screen using 1902 biologically active compounds to identify new molecules that promote proliferation of primary human proximal tubular epithelial cells in vitro.

Results

The primary screen identified 129 compounds that stimulated tubular epithelial cell proliferation. A secondary screen agai

SUBMITTER: Monteiro MB 

PROVIDER: S-EPMC6287872 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature