Whole-Brain Calcium Imaging during Physiological Vestibular Stimulation in Larval Zebrafish.

ABSTRACT: The vestibular apparatus provides animals with postural and movement-related information that is essential to adequately execute numerous sensorimotor tasks. In order to activate this sensory system in a physiological manner, one needs to macroscopically rotate or translate the animal's head, which in turn renders simultaneous neural recordings highly challenging. Here we report on a novel miniaturized, light-sheet microscope that can be dynamically co-rotated with a head-restrained zebrafish larva, enabling controlled vestibular stimulation. The mechanical rigidity of the microscope allows one to perform whole-brain functional imaging with state-of-the-art resolution and signal-to-noise ratio while imposing up to 25° in angular position and 6,000°/s² in rotational acceleration.

SUBMITTER: Migault G 

PROVIDER: S-EPMC6288061 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature